Rabbit anti-MST2/STK3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-467A maps to a region between residues 400 and 450 of human Mammalian Sterile 20-like 2 (Serine/Threonine Kinase 3) using the numbering given in Swiss Prot entry Q13188 (GeneID 6788).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
MST2/STK3 is a member of the sterile 20 (Ste20)-like kinase family that acts upstream of mitogen-activated protein kinase (MAPK) pathways and regulates c-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK). Ste20-like kinases are involved in a variety of cellular processes which include gene transcription, stress responses, cytoskeletal organization and apoptosis. In response to apoptotic signals, MST2/STK3 is cleaved by caspase-3 and mediates apoptosis.
Alternate Names
epididymis secretory sperm binding protein; KB-1458E12.1; KRS1; mammalian STE20-like protein kinase 2; MST2; MST-2; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine-protein kinase 3; serine/threonine-protein kinase Krs-1; STE20-like kinase MST2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.