Rabbit anti-MTA2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-395A maps to a region between residues 500 and 550 of human metastasis-associated gene family, member 2 using the numbering given in Swiss-Prot entry O94776 (GeneID 9219).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MTA2 immobilized on solid support.
The epitope recognized by A300-395A-T maps to a region between residues 500 and 550 of human metastasis-associated gene family, member 2 using the numbering given in Swiss-Prot entry O94776 (GeneID 9219).
Additional Product Information
MTA2 (metastasis-associated protein 2) is a component of the NuRD (nucleosome remodeling and histone deacetylation) complex. MTA2 is highly similar to MTA1 and was identified as a factor differentially expressed in a carcinoma cell line. MTA2 can repress p53-dependent transcriptional activation by enhancing its deacetylation. MTA1 has also been shown to function as a corepressor for ligand-induced estrogen receptor (ER) transactivation. As part of the NuRD complex MTA2 represses ERalpha transactivation activity by modulating the acetylation status of ERalpha.
Alternate Names
metastasis -associated gene 1-like 1; metastasis associated gene family, member 2; metastasis-associated 1-like 1; metastasis-associated protein 2; metastasis-associated protein MTA2; MTA1L1; MTA1-L1 protein; p53 target protein in deacetylase complex; PID
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.