Rabbit anti-MYH14/Myosin-14 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-270A maps to a region between residue 1850 to 1900 of human Myosin-14 using the numbering given in entry Q7Z406.2 (GeneID 79784).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MYH14/Myosin-14 immobilized on solid support.
The epitope recognized by A305-270A-T maps to a region between residue 1850 to 1900 of human Myosin-14 using the numbering given in entry Q7Z406.2 (GeneID 79784).
Additional Product Information
Myosin plays a role in cytokinesis, cell shape, and specialized functions such as secretion and capping [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7Z406].
Alternate Names
DFNA4; DFNA4A; FP17425; MHC16; MYH14 variant protein; MYH17; myosin; Myosin heavy chain 14; myosin heavy chain, non-muscle IIc; myosin, heavy chain 14, non-muscle; myosin, heavy polypeptide 14; myosin-14; NMHC II-C; NMHC-II-C; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C; PNMHH
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.