Rabbit anti-NAA50 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-595A maps to a region between residue 119 to 169 of human N-alpha-acetyltransferase 50, NatE catalytic subunit using the numbering given in entry Q9GZZ1.1 (GeneID 80218).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NAA50 immobilized on solid support.
The epitope recognized by A305-595A-T maps to a region between residue 119 to 169 of human N-alpha-acetyltransferase 50, NatE catalytic subunit using the numbering given in entry Q9GZZ1.1 (GeneID 80218).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
N-alpha-acetyltransferase 50 (NAA50) is a probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9GZZ1].
Alternate Names
hNaa50p; hNAT5; hSAN; MAK3; N-acetyltransferase 13; N-acetyltransferase 13 (GCN5-related); N-acetyltransferase 5; N-acetyltransferase san homolog; N-alpha-acetyltransferase 50; NAT13; NAT13P; NAT5; NAT5P; natE catalytic subunit; N-epsilon-acetyltransferase 50; SAN
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.