Rabbit anti-NCBP2 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00707 maps to a region between residue 106 to 156 of human nuclear cap binding protein subunit 2 using the numbering given in entry NP_031388.2 (GeneID 22916).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NCBP2 immobilized on solid support.
The epitope recognized by IHC-00707-T maps to a region between residue 106 to 156 of human nuclear cap binding protein subunit 2 using the numbering given in entry NP_031388.2 (GeneID 22916).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Nuclear cap binding protein subunit 2 (NCBP2) is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP2 has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay [taken from NCBI Entrez Gene (GeneID: 22916)].
Alternate Names
20 kDa nuclear cap-binding protein; CBC2; CBP20; cell proliferation-inducing gene 55 protein; NCBP 20 kDa subunit; NCBP interacting protein 1; NCBP-interacting protein 1; NIP1; nuclear cap binding protein subunit 2, 20kD; nuclear cap binding protein subunit 2, 20kDa; nuclear cap-binding protein subunit 2; PIG55
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.