Rabbit anti-NCOA5/CIA Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
NCOA5 (Nuclear Receptor Coactivator 5), also known as CIA (Coactivator Independent of AF-2 function), has been shown interact exclusively with estrogen receptor alpha (ERalpha) and beta (ERbeta) to function as a coactivator and enhance transcriptional activation by ER. NCOA5 has also been demonstrated to function as a corepressor of MYC/c-myc in response to 17-beta estradiol.
Alternate Names
bA465L10.6; CIA; coactivator independent of AF-2; NCoA-5; nuclear receptor coactivator 5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.