Rabbit anti-NCoR Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-146A maps to a region between residue 2000 and 2050 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NCoR immobilized on solid support.
The epitope recognized by A301-146A-T maps to a region between residue 2000 and 2050 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611).
Additional Product Information
The nuclear Receptor corepressor protein (NCoR) mediates gene repression by interacting with nuclear receptors such as the thyroid hormone receptor, retinoic acid receptor, progesterone receptor, androgen receptor, estrogen receptor, and glucocorticoids receptor. NCoR mediates repression by associating with SIN3A/B and the histone deacetylases, HDAC1 and HDAC2, and promoting the formation of repressive chromatin structures.
Alternate Names
hN-CoR; N-CoR; N-CoR1; nuclear receptor corepressor 1; PPP1R109; protein phosphatase 1, regulatory subunit 109; thyroid hormone- and retinoic acid receptor-associated corepressor 1; TRAC1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.