Rabbit anti-NEDD4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-253A-T maps to a region between residue 725 and 775 of human Neural Precursor Cell Expressed, Developmentally Down-regulated 4 using the numbering given in entry AAI36606.1 (GeneID 4734).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NEDD4 immobilized on solid support.
The epitope recognized by A303-253A maps to a region between residue 725 and 775 of human Neural Precursor Cell Expressed, Developmentally Down-regulated 4 using the numbering given in entry AAI36606.1 (GeneID 4734).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
NEDD4 (neural precursor cell expressed developmentally down-regulated protein 4) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Independent of its ubiquitin-ligase activity, NEDD4 is involved in the pathway leading to the degradation of VEGFR-2/KDFR [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P46934].
Alternate Names
cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; NEDD4-1; Neural precursor cell expressed developmentally down-regulated protein 4; receptor-potentiating factor 1; RPF1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.