Rabbit anti-NEDD4L Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-514A maps to a region between residue 425 and 475 of human neural precursor cell expressed, developmentally down-regulated 4-like using the numbering given in entry NP_001138439.1 (GeneID 23327).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NEDD4L immobilized on solid support.
The epitope recognized by A302-514A-T maps to a region between residue 425 and 475 of human neural precursor cell expressed, developmentally down-regulated 4-like using the numbering given in entry NP_001138439.1 (GeneID 23327).
Additional Product Information
NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like) functions as an E3 ubiquitin-protein ligase. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. It also promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5 [taken from the Universal Protein Resource (UniProt) wwwuniprotorg/uniprot/Q96PU5].
Alternate Names
E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; hNEDD4-2; NEDD4.2; NEDD4-2; PVNH7; RSP5; ubiquitin-protein ligase Rsp5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.