Rabbit anti-NELFE IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00385 maps to a region between residue 330 and 380 of human negative elongation factor polypeptide E using the numbering given in entry NP_002895.3 (GeneID 7936).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NELFE immobilized on solid support.
The epitope recognized by IHC-00385-T maps to a region between residue 330 and 380 of human negative elongation factor polypeptide E using the numbering given in entry NP_002895.3 (GeneID 7936).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
NELFE (negative elongation factor E) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELF-E is an RNA-binding protein. The RNA binding activity of NELF-E is critical to the function of NELF.
Alternate Names
D6S45; major histocompatibility complex gene RD; negative elongation factor E; negative elongation factor polypeptide E; NELF-E; nuclear protein; RD; RD RNA-binding protein; RDBP; RDP; RNA-binding protein RD
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.