Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR189J]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 275 and 325 of human Neurofilament heavy using the numbering given in entry NP_066554.2 (Gene ID 4744).
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 275 and 325 of human Neurofilament heavy using the numbering given in entry NP_066554.2 (Gene ID 4744).
Additional Product Information
Neurofilaments are type IV intermediate filament heteropolymers composed of light (L), medium (M), and heavy (H) chains. Neurofilaments are a component of the axon cytoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. NF-H is commonly used as a biomarker of neuronal damage [taken from NCBI Entrez Gene (Gene ID: 4744)].
Alternate Names
200 kDa neurofilament protein; CMT2CC; neurofilament heavy polypeptide; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NFH; NF-H
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.