Rabbit anti-NF-kappaB1 (p105) IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00518 maps to a region between residue 918 and 968 of human nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) using the numbering given in entry AAF35232.1 (GeneID 4790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NF-kappaB1 immobilized on solid support.
The epitope recognized by IHC-00518-T maps to a region between residue 918 and 968 of human nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) using the numbering given in entry AAF35232.1 (GeneID 4790).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The NFKB1 gene encodes a 105 kDa protein that can undergo co-translational processing to also yield a 50 kDa protein. The 50 kDa protein is also known as NF-kappa B1/p50 and is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. The 105 kDa product is also known as p105 or I kappa B gamma. p105/I kappa B gamma has been shown to function as an inhibitor protein that interacts with NF-kappa B and inhibit its DNA binding activity. p105/I kappa B gamma is a member of the I kappa B family of proteins which includes I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa B1/p105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. The classical description of the NF-kappa B transcription factor is a transcription factor that reprograms gene expression of the immune system in response to injury, infection, and stress.
Alternate Names
CVID12; DNA-binding factor KBF1; EBP-1; KBF1; NFkappaB; NF-kappaB; NF-kappa-B1; NF-kappabeta; NF-kB1; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105; p50
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.