Rabbit anti-NFX1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-916A maps to a region between residue 1070 and 1120 of human Nuclear Transcription Factor, X-box Binding 1 using the numbering given in entry NP_002495.2 (GeneID 4799).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NFX1 immobilized on solid support.
The epitope recognized by A302-916A-T maps to a region between residue 1070 and 1120 of human Nuclear Transcription Factor, X-box Binding 1 using the numbering given in entry NP_002495.2 (GeneID 4799).
Additional Product Information
NFX1 (nuclear transcription factor, X box-binding protein 1) binds to the X-box motif of MHC class II genes and represses their expression. NFX1 may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 of NFX1 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q12986].
Alternate Names
NFX2; nuclear transcription factor, X box-binding protein 1; TEG-42; Tex42; transcriptional repressor NF-X1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.