Rabbit anti-NMT1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-253A maps to a region between residue 50 to 100 of human N-Myristoyltransferase 1 using the numbering given in entry NP_066565.1 (GeneID 4836).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NMT1 immobilized on solid support.
The epitope recognized by A304-253A-T maps to a region between residue 50 to 100 of human N-Myristoyltransferase 1 using the numbering given in entry NP_066565.1 (GeneID 4836).
Additional Product Information
NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].
Alternate Names
alternative, short form NMT-S; glycylpeptide N-tetradecanoyltransferase 1; long form, NMT-L; myristoyl-CoA:protein N-myristoyltransferase 1; NMT; NMT 1; Peptide N-myristoyltransferase 1; type I N-myristoyltransferase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.