Rabbit anti-NO66 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-199A maps to a region between residue 50 and 100 of human Nucleolar Protein 66 using the numbering given in entry NP_078920.2 (GeneID 79697).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NO66 immobilized on solid support.
The epitope recognized by A303-199A-T maps to a region between residue 50 and 100 of human Nucleolar Protein 66 using the numbering given in entry NP_078920.2 (GeneID 79697).
Additional Product Information
lysine-specific demethylase NO66 (nucleolar protein 66) specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Additionally, NO66, preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2).
Alternate Names
60S ribosomal protein L8 histidine hydroxylase; bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; C14orf169; histone lysine demethylase NO66; hsNO66; JMJD9; lysine-specific demethylase NO66; MAPJD; MYC-associated protein with JmjC domain; NO66; nucleolar protein 66; ribosomal oxygenase 1; ribosomal oxygenase NO66; ROX; up-regulated in lung cancer 2; URLC2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.