Rabbit anti-NOP132 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-722A maps to a region between residue 200 and 250 of human nucleolar protein Nop132 using the numbering given in entry NP_060418.4 (GeneID 55035).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NOP132 immobilized on solid support.
The epitope recognized by A302-722A-T maps to a region between residue 200 and 250 of human nucleolar protein Nop132 using the numbering given in entry NP_060418.4 (GeneID 55035).
Additional Product Information
Nucleolar protein 132 (NOP132) was identified as a protein that interacts with the G protein, RRAG A (RAG A)/GTR1p, a member of the Ras-like small G protein family. NOP132 has been shown to interact mainly with proteins involved in ribosome biogenesis such as DDX47. NOP132 may play a role in recruiting pre-RNA processing proteins to regions of rRNA transcription in the nucleus.
Alternate Names
bA62C3.3; bA62C3.4; C9orf34; NOP132; nucleolar protein 8; nucleolar protein Nop132
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.