Rabbit anti-NRBF2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-852A maps to a region between residue 237 and 287 of human nuclear receptor binding factor 2 using the numbering given in entry NP_110386.2 (GeneID 29982).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NRBF2 immobilized on solid support.
The epitope recognized by A301-852A-T maps to a region between residue 237 and 287 of human nuclear receptor binding factor 2 using the numbering given in entry NP_110386.2 (GeneID 29982).
Additional Product Information
NRBF2 (nuclear receptor binding factor-2) was originally identified in a yeast two-hybrid screen for factors that interact with the peroxisome proliferator-activated receptor alpha (PPARalpha). In a second study, NRBF2 was also identified in a screen of a keratinocyte cDNA library and named COPR (comodulator of PPAR and RXR). In this study, NRBF2 was proposed to function as a modulator of transcriptional activity that functions to decrease rather than completely repress nuclear-receptor mediated gene expression.
Alternate Names
Comodulator of PPAR and RXR; comodulator of PPAR and RXR 1; comodulator of PPAR and RXR 2; COPR; COPR1; COPR2; NRBF-2; nuclear receptor-binding factor 2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.