Rabbit anti-NUDCD3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-865A-T maps to a region between residue 100 to 150 of human NudC domain-containing protein 3 using the numbering given in entry Q8IVD9.3 (GeneID 23386).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUDCD3 immobilized on solid support.
The epitope recognized by A304-865A maps to a region between residue 100 to 150 of human NudC domain-containing protein 3 using the numbering given in entry Q8IVD9.3 (GeneID 23386).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
NudC domain-containing protein 3 (NUDCD3) functions to maintain the stability of dynein intermediate chain. Depletion NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase, and levels of the protein increase after the G1/S transition [taken from NCBI Entrez Gene (Gene ID: 23386)].
Alternate Names
nudC domain-containing protein 3; NudCL; NudC-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.