Rabbit anti-NUMB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-714A maps to a region between residue 375 and 425 of human NUMB homolog using the numbering given in entry NP_001005743.1 (GeneID 8650).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUMB immobilized on solid support.
The epitope recognized by A301-714A-T maps to a region between residue 375 and 425 of human NUMB homolog using the numbering given in entry NP_001005743.1 (GeneID 8650).
Additional Product Information
NUMB plays an essential role in the determination of cell fate and development and appears to be especially important to sensory organogenesis and neurogenesis. NUMB is a notch1-interacting protein that antagonizes the notch1 signaling pathway. In the cell, NUMB is asymmetrically localized. The polarized distribution of NUMB in the cell is fundamental to its function of promoting asymmetric cell division for cell fate specification. Numb is also implicated to function as an endocytic protein, as it interacts with EPS15, a protein involved in endocytosis.
Alternate Names
c14_5527; C14orf41; h-Numb; numb homolog; protein numb homolog; Protein S171; S171
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.