Rabbit anti-NUPL Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-792A maps to a region between residue 512 and 562 of human nucleoporin-like protein RIP using the numbering given in entry NP_004495.2 (GeneID 3267).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUPL immobilized on solid support.
The epitope recognized by A301-792A-T maps to a region between residue 512 and 562 of human nucleoporin-like protein RIP using the numbering given in entry NP_004495.2 (GeneID 3267).
Additional Product Information
NUPL or hRIP (human Rev interacting protein) was originally identified in a yeast two-hybrid screen for proteins that interact with the Rev protein of the human immunodeficiency virus type 1 (HIV-1). Structurally, hRIP contains an ARFGAP (ARF GTPase-activating protein) domain. hRIP was found to have homology with nucleoporins; thus it is also referred to as nucleoporin-like protein (NUPL) RIP. hRIP is essential to HIV-1 replication but not for cell viability, and it appears to be important for the export of HIV-1 RNA from the perinuclear region to the cytoplasm.
Alternate Names
arf-GAP domain and FG repeat-containing protein 1; arf-GAP domain and FG repeats-containing protein 1; HIV-1 Rev-binding protein; HRB; hRIP, Rev interacting protein; nucleoporin-like protein RIP; RAB; Rab, Rev/Rex activation domain-binding protein; rev/Rex activation domain-binding protein; rev-interacting protein; RIP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.