Rabbit anti-Occludin Recombinant Monoclonal Antibody [BLR126H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 472 and the C-terminus (residue 522) of human occludin using the numbering given in entry NP_001192183.1 (Gene ID 100506658).
Additional Product Information
Occludin may play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16625].
Alternate Names
BLCPMG; occludin; phosphatase 1, regulatory subunit 115; PPP1R115; PTORCH1; tight junction protein occludin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.