Rabbit anti-p190RhoGEF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-930A maps to a region between residue 1655 and 1705 of human 190 kDa, Rho-Guanine Nucleotide Exchange Factor using the numbering given in entry NP_001171164.1 (GeneID 64283).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to p190RhoGEF immobilized on solid support.
The epitope recognized by A303-930A-T maps to a region between residue 1655 and 1705 of human 190 kDa, Rho-Guanine Nucleotide Exchange Factor using the numbering given in entry NP_001171164.1 (GeneID 64283).
Additional Product Information
Rho guanine nucleotide exchange factor 28 (p190RhoGEF) Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. p190RhoGEF functions in axonal branching, synapse formation and dendritic morphogenesis. p190RhoGEF also functions in focal adhesion formation, cell motility and B-lymphocytes activation. It may regulate NEFL expression and aggregation and play a role in apoptosis (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
Alternate Names
190 kDa guanine nucleotide exchange factor; p190RHOGEF; p190-RhoGEF; RGNEF; Rho guanine nucleotide exchange factor; Rho guanine nucleotide exchange factor (GEF) 28; rho guanine nucleotide exchange factor 28; Rho interacting protein 2; rho-guanine nucleotide exchange factor; RIP2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.