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Rabbit anti-PACSIN3 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PACSIN3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 300 and 350
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PACSIN3 immobilized on solid support.

The epitope recognized by A301-981A-T maps to a region between residue 275 and 325 of human protein kinase C and casein kinase substrate in neurons 3 using the numbering given in entry NP_057307.2 (GeneID 29763).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PACSIN3 immobilized on solid support.

The epitope recognized by A301-981A maps to a region between residue 275 and 325 of human protein kinase C and casein kinase substrate in neurons 3 using the numbering given in entry NP_057307.2 (GeneID 29763).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

PACSIN3 (protein kinase C and casein kinase substrate in neurons protein 3) is a member of the PACSIN family, a group of adaptor protein that regulate vesicle trafficking via cytoskeletal alterations. PACSIN3 is primarily expressed in skeletal muscle and heart and has been shown to be specifically involved in the trafficking of GLUT 1 in adipocytes.

29763
PACSIN3
protein kinase C and casein kinase substrate in neurons 3
Q9UKS6
Protein kinase C and casein kinase substrate in neurons protein 3

Alternate Names

protein kinase C and casein kinase substrate in neurons protein 3; SDPIII; SH3 domain-containing protein 6511; syndapin III

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.