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Rabbit anti-PALB2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PALB2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 700 and 750
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PALB2 immobilized on solid support.

The epitope recognized by A301-247A maps to a region between residue 675 and 725 of human partner and localizer of BRCA2 using the numbering given in entry NP_078951.2 (GeneID 79728).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PALB2 immobilized on solid support.

The epitope recognized by A301-247A-T maps to a region between residue 675 and 725 of human partner and localizer of BRCA2 using the numbering given in entry NP_078951.2 (GeneID 79728).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

PALB2 (partner and localizer of BRCA2) interacts with BRCA2 and promotes BRCA2 localization and stability. PALB2 is also known as FANCN. Bi-allelic mutations in PALB2 result in Fanconi anemia disorder and breast cancer susceptibility. At the cellular level, PALB2 is important to the DNA damage response and chromosome stability and is essential to BRCA2 function.

79728
PALB2
partner and localizer of BRCA2
Q86YC2
Partner and localizer of BRCA2

Alternate Names

FANCN; mutant partner and localizer of BRCA2; partner and localizer of BRCA2; PNCA3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.