Rabbit anti-PARG Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-405A-T maps to a region between residue 250 to 300 of human Poly using the numbering given in entry Q86W56.1 (GeneID 8505).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PARG immobilized on solid support.
The epitope recognized by A305-405A maps to a region between residue 250 to 300 of human Poly using the numbering given in entry Q86W56.1 (GeneID 8505).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. PARG is found in many tissues and may be subject to proteolysis generating smaller, active products [taken from NCBI Entrez Gene (Gene ID: 8505)].
Alternate Names
mitochondrial poly(ADP-ribose) glycohydrolase; PARG99; poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.