Rabbit anti-PCAF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-666A maps to a region between residue 782 and 832 of human p300/CBP-associated factor using the numbering given in entry NP_003875.3 (GeneID 8850).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PCAF immobilized on solid support.
The epitope recognized by A301-666A-T maps to a region between residue 782 and 832 of human p300/CBP-associated factor using the numbering given in entry NP_003875.3 (GeneID 8850).
Additional Product Information
PCAF (P300/CBP-associated factor) is a histone acetyltransferase (HAT) identified as a factor that associates with P300/CBP, a transcriptional coactivator of nuclear hormone receptors and other transcription factors.
Alternate Names
CAF; CREBBP-associated factor; histone acetylase PCAF; histone acetyltransferase KAT2B; histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; Lysine acetyltransferase 2B; P/CAF; p300/CBP-associated factor; PCAF
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.