Rabbit anti-PCSK9 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-107A-T maps to a region between residue 450 to 500 of human Proprotein convertase subtilisin/kexin type 9 using the numbering given in entry Q8NBP7.3 (GeneID 255738).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PCSK9 immobilized on solid support.
The epitope recognized by A305-107A maps to a region between residue 450 to 500 of human Proprotein convertase subtilisin/kexin type 9 using the numbering given in entry Q8NBP7.3 (GeneID 255738).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The gene PCSK9, also known as NARC1, encodes for proprotein convertase subtilisin/kexin type 9. A member of the subtilisin-like proprotein convertase family, this protein is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia [taken from NCBI Entrez Gene (Gene ID: 255738)].
Alternate Names
convertase subtilisin/kexin type 9 preproprotein; FH3; HCHOLA3; LDLCQ1; NARC1; NARC-1; neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC9; Proprotein convertase 9; proprotein convertase subtilisin/kexin type 9; subtilisin/kexin-like protease PC9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.