Rabbit anti-PF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-647A maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PF1 immobilized on solid support.
The epitope recognized by A301-647A-T maps to a region between residue 954 and 1004 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
Additional Product Information
PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks.
Alternate Names
PF1; PHD factor 1; PHD finger protein 12; PHD zinc finger transcription factor
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.