Rabbit anti-Phospho RelA (S536) IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00524 maps to a region of human v-rel reticuloendotheliosis viral oncogene homolog A surrounding serine 536 when serine 536 is phosphorylated using the numbering given in entry NP_068810.2 (GeneID 5970).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Phospho RelA (S536) immobilized on solid support.
The epitope recognized by IHC-00524-T maps to a region of human v-rel reticuloendotheliosis viral oncogene homolog A surrounding serine 536 when serine 536 is phosphorylated using the numbering given in entry NP_068810.2 (GeneID 5970).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
RelA is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. Each Rel member bears a characteristic 300 amino acid N-terminal Rel homology domain (RHD) that functions to mediate DNA binding, dimerization, nuclear localization, and inhibitor association.
Alternate Names
NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.