Rabbit anti-Phospho SMC1 (S957) Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-147A maps to a region of human SMC1 (structural maintenance of chromosomes 1-like 1) surrounding serine 957 using the numbering given in entry NP_006297.2 (Gene ID 8243) when the serine residue is phosphorylated.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Phospho SMC1 (S957) immobilized on solid support.
The epitope recognized by A304-147A-T maps to a region of human SMC1 (structural maintenance of chromosomes 1-like 1) surrounding serine 957 using the numbering given in entry NP_006297.2 (Gene ID 8243) when the serine residue is phosphorylated.
Additional Product Information
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
Alternate Names
CDLS2; DXS423E; epididymis secretory sperm binding protein; SB1.8; segregation of mitotic chromosomes 1; SMC protein 1A; SMC1; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; SMC-1A; SMC1alpha; SMC-1-alpha; SMC1L1; SMCB; structural maintenance of chromosomes protein 1A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.