Rabbit anti-PHRF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-044A-T maps to a region between residue 1400 and 1450 of human PHD and ring finger domains 1 using the numbering given in entry NP_065952.1 (GeneID 57661). Antibody was affinity purified using an epitope specific to PHRF1 immobilized on solid support.
The epitope recognized by A302-044A maps to a region between residue 1400 and 1450 of human PHD and ring finger domains 1 using the numbering given in entry NP_065952.1 (GeneID 57661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
PHD and RING finger domain-containing protein 1 is an uncharacterized protein that contains a PHD-type zinc finger and a RING-type zinc finger.
Alternate Names
CTD-binding SR-like protein rA9; PHD and RING finger domain-containing protein 1; PPP1R125; protein phosphatase 1, regulatory subunit 125; RNF221
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.