Rabbit anti-PI4KB Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-733A maps to a region between residue 1 and 50 of human Phosphatidylinositol 4-Kinase, Catalytic, beta using the numbering given in entry CAA09495.1 (GeneID 5298).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PI4KB immobilized on solid support.
The epitope recognized by A303-733A-T maps to a region between residue 1 and 50 of human Phosphatidylinositol 4-Kinase, Catalytic, beta using the numbering given in entry CAA09495.1 (GeneID 5298).
Additional Product Information
Phosphatidylinositol 4-kinase beta (PI4KB) phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). PI4KB may regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation, and may be involved in Golgi-to-plasma membrane trafficking [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9UBF8].
Alternate Names
NPIK; phosphatidylinositol 4-kinase beta; phosphatidylinositol 4-kinase, catalytic, beta; phosphatidylinositol 4-kinase, wortmannin-sensitive; PI4K92; PI4KBETA; PI4K-BETA; PI4KIIIBETA; PIK4CB; PtdIns 4-kinase beta; type III phosphatidylinositol 4-kinase beta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.