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Rabbit anti-PICALM/CALM Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PICALM/CALM
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 602 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PICALM/CALM immobilized on solid support.

The epitope recognized by A305-301A maps to a region between residue 602 to 652 of human Phosphatidylinositol-binding clathrin assembly protein using the numbering given in entry Q13492.2 (GeneID 8301).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PICALM/CALM immobilized on solid support.

The epitope recognized by A305-301A-T maps to a region between residue 602 to 652 of human Phosphatidylinositol-binding clathrin assembly protein using the numbering given in entry Q13492.2 (GeneID 8301).
Country of Origin
USA

Additional Product Information

PICALM/CALM is a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. PICALM/CALM is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction [taken from NCBI Entrez Gene (Gene ID: 8301)].

8301
PICALM
phosphatidylinositol binding clathrin assembly protein
Q13492
Phosphatidylinositol-binding clathrin assembly protein

Alternate Names

CALM; clathrin assembly lymphoid myeloid leukemia protein; CLTH; LAP; phosphatidylinositol-binding clathrin assembly protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).