Rabbit anti-PKC-eta Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-236A maps to a region between residue 633 to 683 of human Protein Kinase C, Eta using the numbering given in entry NP_006246.2 (GeneID 5583).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PKC-eta immobilized on solid support.
The epitope recognized by A304-236A-T maps to a region between residue 633 to 683 of human Protein Kinase C, Eta using the numbering given in entry NP_006246.2 (GeneID 5583).
Additional Product Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC-eta is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. PKC-eta can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene [taken from NCBI Entrez Gene (Gene ID: 5583)].
Alternate Names
nPKC-eta; PKCL; PKC-L; PRKCL; protein kinase C eta type
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.