Rabbit anti-PLEKHA1/TAPP1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-250A-T maps to a region between residue 354 to 404 of human Pleckstrin homology domain-containing family A member 1 using the numbering given in entry Q9HB21.2 (GeneID 59338).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PLEKHA1/TAPP1 immobilized on solid support.
The epitope recognized by A305-250A maps to a region between residue 354 to 404 of human Pleckstrin homology domain-containing family A member 1 using the numbering given in entry Q9HB21.2 (GeneID 59338).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Pleckstrin homology domain containing A1 (PLECKHA1) binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. PLECKHA1 may recruit other proteins to the plasma membrane. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HB21].
Alternate Names
PH domain-containing family A member 1; pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1; pleckstrin homology domain-containing family A member 1; tandem PH domain-containing protein 1; TAPP1; TAPP-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.