Rabbit anti-PMVK Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-576A maps to a region between residue 142 to 192 of human Phosphomevalonate kinase using the numbering given in entry Q15126.3 (GeneID 10654).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PMVK immobilized on solid support.
The epitope recognized by A305-576A-T maps to a region between residue 142 to 192 of human Phosphomevalonate kinase using the numbering given in entry Q15126.3 (GeneID 10654).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
PMVK is a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. PMVK protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis [taken from NCBI Entrez Gene (Gene ID: 10654)].
Alternate Names
hPMK; HUMPMKI; phosphomevalonate kinase; PMK; PMKA; PMKASE; POROK1; testis tissue sperm-binding protein Li 95mP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.