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Rabbit anti-Pol Iota Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Pol Iota
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 690 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Formulation Change
Country of Origin
USA

Additional Product Information

Pol Iota is a DNA polymerase involved in DNA repair and translesion DNA synthesis. Pol Iota is a member of the Rad30 family of DNA polymerases which exhibit low fidelity when replicating undamaged DNA, and in this context may play a role in the hypermutation of immunoglobulin genes. Pol Iota is also known as RAD30B and is a paralog of RAD30A, also known as Pol eta.

11201
POLI
DNA polymerase iota
Q9UNA4
DNA polymerase iota

Alternate Names

DNA polymerase iota; eta2; polymerase (DNA directed) iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD30 homolog B; RAD30B; RAD3OB

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.