Rabbit anti-Pol Iota Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-304A maps to a region between residue 690 and 715 of human DNA polymerase iota using the numbering given in entry NP_009126.1 (GeneID 11201).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pol Iota immobilized on solid support.
The epitope recognized by A301-304A-T maps to a region between residue 690 and 715 of human DNA polymerase iota using the numbering given in entry NP_009126.1 (GeneID 11201).
Additional Product Information
Pol Iota is a DNA polymerase involved in DNA repair and translesion DNA synthesis. Pol Iota is a member of the Rad30 family of DNA polymerases which exhibit low fidelity when replicating undamaged DNA, and in this context may play a role in the hypermutation of immunoglobulin genes. Pol Iota is also known as RAD30B and is a paralog of RAD30A, also known as Pol eta.
Alternate Names
DNA polymerase iota; eta2; polymerase (DNA directed) iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD30 homolog B; RAD30B; RAD3OB
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.