Rabbit anti-Pol kappa Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-976A-T maps to a region between residue 775 and 825 of human DNA polymerase kappa using the numbering given in entry NP_057302.1 (GeneID 51426).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Pol kappa immobilized on solid support.
The epitope recognized by A301-976A maps to a region between residue 775 and 825 of human DNA polymerase kappa using the numbering given in entry NP_057302.1 (GeneID 51426).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
DNA polymerase kappa (Pol kappa) is a DNA polymerase specifically involved in DNA repair. It is critical to translesion synthesis and can bypass DNA damage that would typically arrest high-fidelity DNA synthesis.
Alternate Names
DINB protein; DINB1; DINP; DNA polymerase kappa; POLQ; polymerase (DNA directed) kappa
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.