Rabbit anti-PolA1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-851A maps to a region between residue 1175 and 1225 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PolA1 immobilized on solid support.
The epitope recognized by A302-851A-T maps to a region between residue 1175 and 1225 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Additional Product Information
DNA polymerase alpha catalytic subunit (PolA1) is the catalytic subunit of the DNA polymerase alpha complex. The DNA polymerase alpha complex is composed of PolA1, PolA2, and two primase subunits, PRIM1 and PRIM2.
Alternate Names
DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; NSX; p180; POLA; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.