Rabbit anti-PolD1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-006A maps to a region between residue 850 to 900 of human Polymerase (DNA directed), Delta 1, Catalytic Subunit 125kDa using the numbering given in entry NP_002682.2 (GeneID 5424).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PolD1 immobilized on solid support.
The epitope recognized by A304-006A-T maps to a region between residue 850 to 900 of human Polymerase (DNA directed), Delta 1, Catalytic Subunit 125kDa using the numbering given in entry NP_002682.2 (GeneID 5424).
Additional Product Information
DNA polymerase delta catalytic subunit (PolD1) is the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair [taken from NCBI Entrez Gene (Gene ID: 5424)].
Alternate Names
CDC2; CDC2 homolog; CRCS10; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; MDPL; POLD; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.