Rabbit anti-PolH Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-231A maps to a region between residue 690 and 713 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PolH immobilized on solid support.
The epitope recognized by A301-231A-T maps to a region between residue 690 and 713 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
Additional Product Information
PolH is a DNA polymerase specifically involved in DNA repair and somatic hypermutation. Defects in PolH result in a variant type of xeroderma pigmentosum, a disease characterized by sunlight hypersensitivity, increased incidence of cancer, and neurological abnormalities.
Alternate Names
DNA polymerase eta; polymerase (DNA directed), eta; polymerase (DNA) eta; RAD30; RAD30 homolog A; RAD30A; xeroderma pigmentosum variant type protein; XPV; XP-V
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.