Rabbit anti-POLR3D Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-295A maps to a region between residue 175 and 225 of human DNA-directed RNA polymerase III subunit D using the numbering given in entry NP_001713.2 (GeneID 661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to POLR3D immobilized on solid support.
The epitope recognized by A302-295A-T maps to a region between residue 175 and 225 of human DNA-directed RNA polymerase III subunit D using the numbering given in entry NP_001713.2 (GeneID 661).
Additional Product Information
RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA.
Alternate Names
BN51 (BHK21) temperature sensitivity complementing; BN51T; DNA-directed RNA polymerase III 47 kDa polypeptide; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III subunit RPC4; polymerase (RNA) III (DNA directed) polypeptide D, 44kDa; polymerase (RNA) III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RNA polymerase III subunit C4; RPC4; RPC53; RPC53 homolog; temperature sensitive complementation, cell cycle specific, tsBN51; TSBN51
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.