Rabbit anti-PPP2R1A Antibody, Affinity Purified
Product Details
Specifications
The epitope recognized by A300-963A maps to a region between residue 540 and the C-terminus (residue 588) of human protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform using the numbering given in entry NP_055040.2 (GeneID 5518).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPP2R1A immobilized on solid support.
The epitope recognized by A300-963A-T maps to a region between residue 540 and the C-terminus (residue 588) of human protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform using the numbering given in entry NP_055040.2 (GeneID 5518).
Additional Product Information
PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. It is a trimeric holoenzyme that is comprised of a catalytic, structural, and regulatory subunit. PPP2R1A is the alpha isoform of the 65kd structural subunit of PP2A. The PP2A catalytic subunit complexed with PPP2R1A can associate with various regulatory subunits to form trimeric holoenzymes with distinct activities.
Alternate Names
medium tumor antigen-associated 61 KDA protein; MRD36; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2AA; PP2AAALPHA; PP2A-Aalpha; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase 2, regulatory subunit A, alpha; protein phosphatase 2A structural subunit A, alpha isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; testicular secretory protein Li 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.