Rabbit anti-PPP4R3 Alpha IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00469 maps to a region between residue 770 and the C-terminus (residue 820) of human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) using the numbering given in entry NP_115949.3 (GeneID 55671).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPP4R3 Alpha immobilized on solid support.
The epitope recognized by IHC-00469-T maps to a region between residue 770 and the C-terminus (residue 820) of human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) using the numbering given in entry NP_115949.3 (GeneID 55671).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
PPP4R3 is a binding partner of the PPP4C catalytic subunit of the PP4 serine/threonine protein phosphatase. PPP4C is structurally related to PP2AC but does not associate with the same regulatory subunits. PPP4C associates with either the PPP4R1 or PPP4R2 regulatory subunit. In addition to PPP4R1 and PPP4R2, PPP4R3 has also been identified as a regulator of PPP4C function. PP4R3 functions with PP4C in the control of recovery from DNA damage. PP4R3 may function by targeting PPP4 to sites of DNA damage via an interaction with Rad53 (CHK2). PP4R3 is the human homolog of the Dictylostelium SMEK (suppressor of MEK). SMEK has been shown to be involved in cell polarization and chemotaxis via an involvement in the MEK1/ERK1 signaling pathway. SMEK also functions by controlling PPP4C localization to nucleus.
Alternate Names
FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1; SMEK homolog 1, suppressor of mek1; SMEK1; smk1; smk-1
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.