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Rabbit anti-PRDX6 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PRDX6
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 50 and 100
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Pig, Bovine
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PRDX6 immobilized on solid support.

The epitope recognized by A305-316A maps to a region between residue 50 to 100 of human Peroxiredoxin-6 using the numbering given in entry P30041.3 (GeneID 9588).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PRDX6 immobilized on solid support.

The epitope recognized by A305-316A-T maps to a region between residue 50 to 100 of human Peroxiredoxin-6 using the numbering given in entry P30041.3 (GeneID 9588).
Country of Origin
USA

Additional Product Information

Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family. PRDX6 is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury [taken from NCBI Entrez Gene (Gene ID: 9588)].

9588
PRDX6
peroxiredoxin 6
P30041
Peroxiredoxin-6

Alternate Names

1-Cys; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; AOP2; epididymis secretory sperm binding protein Li 128m; HEL-S-128m; liver 2D page spot 40; non-selenium glutathione peroxidase; NSGPx; p29; peroxiredoxin-6; PRX; red blood cells page spot 12

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.