Rabbit anti-Prefoldin 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-795A maps to a region between residue 72 and 122 of human Prefoldin Subunit 1 using the numbering given in entry NP_002613.2 (GeneID 5201).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Prefoldin 1 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils [taken from NCBI Entrez Gene (Gene ID: 5201)].
Alternate Names
PDF; PFD1; prefoldin 1; prefoldin subunit 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.