Rabbit anti-PRKAR1A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-683A maps to a region between residue 1 and 50 of human Protein Kinase, cAMP-Dependent, Regulatory, Type I, Alpha using the numbering given in entry NP_002725.1 (GeneID 5573).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PRKAR1A immobilized on solid support.
The epitope recognized by A303-683A-T maps to a region between residue 1 and 50 of human Protein Kinase, cAMP-Dependent, Regulatory, Type I, Alpha using the numbering given in entry NP_002725.1 (GeneID 5573).
Additional Product Information
cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) is the regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. PRKAR1A is also known as tissue-specific extinguisher 1 (TSE1); PRKAR1A was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in PRKAR1A cause Carney complex (CNC), a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P10644].
Alternate Names
ACRDYS1; ADOHR; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; Carney complex type 1; CNC; CNC1; epididymis secretory sperm binding protein; PKR1; PPNAD1; PRKAR1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha; tissue-specific extinguisher 1; TSE1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.