Rabbit anti-PSMD11 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-751A maps to a region between residue 372 and 422 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSMD11 immobilized on solid support.
The epitope recognized by A302-751A-T maps to a region between residue 372 and 422 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Additional Product Information
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. PSMD11 is a non-ATPase subunit of the 19S regulator.5, S9, Rpn6, and p44.5 [taken from NCBI Entrez Gene (GeneID: 5717)].
Alternate Names
26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit p44.5; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; p44.5; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; Rpn6; S9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.