Rabbit anti-PSMD4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-855A maps to a region between residue 250 and 300 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 4 using the numbering given in entry NP_002801.1 (GeneID 5710).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PSMD4 immobilized on solid support.
The epitope recognized by A303-855A-T maps to a region between residue 250 and 300 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 4 using the numbering given in entry NP_002801.1 (GeneID 5710).
Additional Product Information
PSMD4 [proteasome (prosome, macropain) 26S subunit, non-ATPase, 4] is a non-ATPase subunit of the 19S regulator lid. The 19S regulator and 20S core complexes make up the 26S proteasome which is a multicatalytic proteinase complex with a highly ordered structure. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides [taken from NCBI Entrez Gene (Gene ID: 5710)].
Alternate Names
26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; AF-1; angiocidin; antisecretory factor 1; ASF; MCB1; multiubiquitin chain-binding protein; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; pUB-R5; Rpn10; S5A; S5a/antisecretory factor protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.